Research Paper Volume 12, Issue 1 pp 866—883

Prognostic value of epithelial-mesenchymal transition markers in clear cell renal cell carcinoma

Figure 6. Module analysis and functional annotations of the six EMT related gene in silico. Protein-protein interaction (PPI), activation and indirect relation were predicted and displayed in association with sig EMT related genes (A). PPI network derived from active interaction sources was detailed illustrated with required interaction score equal 0.400 (B). GO and KEGG functional annotations analysis of CDH1, CDH2, SNAI1, SNAI2, VIM, TWIST1 was enriched in hemophilic cell adhesion and cell-cell adhesion of biologic process, adherens junction, anchoring junction and cell junction of cellular component, RPTP-like protein binding, phosphatase binding, protein phosphatase binding and enzyme binding of molecular function. Participating upstream or downstream signaling pathways enrichment include adherens junction, cell adhesion molecules (CAMs) of KEGG pathways (C). Hierarchical partitioning using transcriptional expression profiles of CDH1, CDH2, SNAI1, SNAI2, VIM, TWIST1 from FUSCC cohort (D). Hierarchical partitioning using transcriptional expression profiles of six hub genes from TCGA cohort was performed in the heat map (E).