Figure 1. 40% CR for 6 months (14 to 20 months old) delays age-related alterations in aging liver. Levels of (A) IL-6 and (B) insulin in serum were measured by ELISA. Levels of (C) MDA, (D) SOD, (E) serum TG, (F) liver TG, (G) serum TC, and (H) liver TC were measured using commercial kits. (I) Lipid droplet accumulation was assessed via Oil Red O staining. (J) Western blotting was used to determine levels of senescence markers (K) β-gal and (L) p16. Results are shown as the mean ± SD of eight animals per group. *p < 0.05 compared with the control group.