Figure 2. 40% CR for 6 months (14 to 20 months old) upregulates levels of FMO3 in liver and enhances mTOR-regulated autophagy. (A) Representative western blots of liver lysates from each group of mice, probed with the indicated antibodies. GADPH was used as the internal housekeeping protein control. Image analysis of (B) FMO3, (C) LC3, (D) p62, (E) p-mTOR/mTOR, and (F) p-S6K/S6K was performed using densitometry. Results are shown as the mean ± SD of eight animals per group. *p < 0.05 compared with the control group.