Figure 4. FMO3 overexpression inhibits mTOR signaling and induces autophagy. (A) Representative western blots of liver lysates from each group of mice, probed with the indicated antibodies. GADPH was used as the internal housekeeping protein control. Image analysis of (B) LC3, (C) p62, (D) p-mTOR/mTOR, and (E) p-S6K/S6K was performed using densitometry. Results are shown as the mean ± SD of eight animals per group. *p < 0.05 compared with the control group; #p < 0.05 compared with the CR group; &p < 0.05 compared with the FMO3 overexpression group.