Research Paper Volume 12, Issue 1 pp 931—944

Upregulation of flavin-containing monooxygenase 3 mimics calorie restriction to retard liver aging by inducing autophagy

Figure 5. Bafilomycin A1 treatment suppresses mTOR-regulated autophagy. (A) Representative western blots of liver lysates from each group of mice, probed with the indicated antibodies. GADPH was used as the internal housekeeping protein control. Image analysis of (B) LC3, (C) p62, (D) p-mTOR/ mTOR, and (E) p-S6K/S6K was performed using densitometry. (F) Autophagosomes were observed through an electron microscope (black arrows). Results are shown as the mean ± SD of eight animals per group. *p < 0.05 compared with the control group; #p < 0.05 compared with the FMO3 overexpression group.