Research Paper Volume 12, Issue 4 pp 3126—3139

LncRNA AK045171 protects the heart from cardiac hypertrophy by regulating the SP1/MG53 signalling pathway

Figure 6. AK045171 inhibited the proliferation of cardiac fibroblasts. (A) MTT assays were used to evaluate cardiac fibroblast proliferation. (B) EDU staining was performed to detect the proliferation of cardiac fibroblasts. qPCR was used to detect the expression of fibrosis biomarkers, including (C) collagen I (D) collagen III and (E) MMP-9. (F) Western blotting was carried out to investigate collagen I, collagen III and MMP-9 protein expression. *p<0.05 vs control group; #p<0.05 vs MS+vector group.