Figure 8. Co-regulation of apoptosis and pyroptosis pathways by XIST and SMAD2. (A, B) Western blot analysis of SMAD2, p53, NLRP3, and apoptosis- and pyroptosis-related proteins in NSCLC cells. (C) ChIP assay results indicating reduced interaction of SMAD2 with p53 and NLRP3 following XIST overexpression. (D) Luciferase activity assay showing that SMAD2 knockdown reduces the transcription of p53 and NLRP3. (E) Schematic diagram depicting the potential mechanism mediating XIST-dependent DDP chemoresistance in NSCLC cells.