Figure 5. P66shc was involved in catalpol-mediated protective effects. (A) P66shc protein expression in NAFLD mice. (B) Cytochrome C (Cyto C) protein expression in NAFLD mice. (C) P66shc protein expression in PA- treated hepG2 cells. (D) Cyto C protein expression in PA-treated hepG2 cells. (E) P66shc protein expression was down-regulated by its specific siRNA. (F) JC-1 staining. (G) ROS level. (H) Nile red staining. (I) Tunel staining (Tunel positive cells were stained in green; DAPI was stained to show nuclei). The results are the mean± SD (n=10), **P< 0.05 vs. Control group, ##P < 0.05 vs. WD or PA group.