Research Paper Volume 12, Issue 3 pp 2101—2122

CSF1R inhibitor PLX5622 and environmental enrichment additively improve metabolic outcomes in middle-aged female mice

Figure 3. Microglial depletion in response to PLX5622. (AD) Representative Iba1 immunohistochemistry of medial hypothalamus, in (A) SE PLX(-), (B) SE PLX(+), (C) EE PLX(-), and (D) EE PLX(+). (E) Microglia cell count within hypothalamus. Scale bar in A is 100 μm. (E) n=4 per group. ****p<0.0001. Values are means ± SEM. Statistical analyses are shown in Supplementary File 1.