Figure 3. Microglial depletion in response to PLX5622. (A–D) Representative Iba1 immunohistochemistry of medial hypothalamus, in (A) SE PLX(-), (B) SE PLX(+), (C) EE PLX(-), and (D) EE PLX(+). (E) Microglia cell count within hypothalamus. Scale bar in A is 100 μm. (E) n=4 per group. ****p<0.0001. Values are means ± SEM. Statistical analyses are shown in Supplementary File 1.