Figure 5. Adipose tissue response to PLX5622 and environmental enrichment in middle-aged mice. (A) Adipose tissue macrophages (ATM) from gonadal white adipose tissue. (B) ATM polarization, including CD11c+, CD206+ cells. (C) Representative flow diagram of ATM CD206+ subset depletion. (D) Representative flow diagram of F4/80low, CD11blow population depletion. (A–C) n=5-6 per group. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Values are means ± SEM. Statistical analyses are shown in Supplementary File 1.