Figure 4. FAIM2 is a target of miR-193a-3p, and FAIM2 could be inhibited by miR-193a-3p in HUVECs. (A) The presumptive binding site of miR-193a-3p in the 3′-UTR of FAIM2. (B) Luciferase reporter assay. We cotransfected HUVECs with wild-type or mutant FAIM2 3′-UTR reporters and miR-193a-3p mimics or corresponding control. (C–E) HUVECs were transfected with miR-193a-3p mimics or corresponding control. mRNA and protein expressions of FAIM2 were determined by western blot. n = 3. (Data are presented as the mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001, and ****P < 0.0001).