Figure 6. Effect of BC on the BPH-related and apoptosis related protein and mRNA expression in WPMY-1 cells. (A) Effect of BC on the cell viability in WPMY-1 cells. WPMY-1 cells were treated with 3.13 to 200 μM of BC for 24 h. (B) Inhibitory effect of BC on cell proliferation in DHT-stimulated WPMY-1 cells. The cells were treated with 10 nM DHT, with or without 6.25–200 μM of BC for 24h. (C–E) WPMY-1 cells were stimulated with 10 nM DHT for 24h, with or without BC (25, 50 μM). (B) The mRNA level of AR, PSA, PCNA, Bax, Bcl-xL and Bcl-2 were quantificated using RT-PCR in DHT-stimulated WPMY-1 cells. Protein levels of (C) AR, PSA, PCNA (D) Bax, Bcl-xL and Bcl-2 are determined using western blotting in DHT-stimulated WPMY-1 cells. β-actin are used for internal control. Results are represented as mean ± SD. P value ### = P < 0.001 versus vehicle group; * = P < 0.05, ** = P <0.01, *** = P < 0.001 versus the DHT-stimulated group.