Figure 1. P3H4 is highly expressed in BC. (A)The boxplot of P3H4 mRNA level. Red and gray boxes represent bladder urothelial carcinoma (BLCA) tissue and normal bladder tissue, respectively. The data came from the GEPIA database. Disease free survival (B) and overall survival (C) percentage of BLCA patients with high or low P3H4 expression. (D) P3H4 expression in bladder cacner (BC) tissues and adjacent normal tissues was examined by Immunohistochemical (IHC) analysis. After siRNA targeting P3H4 (siP3H4) was transfected into EJ and T24 cells, P3H4 mRNA (E) and protein (F and G) expression were detected by RT-qPCR and western blot, cell proliferation (H and I) were measured by CCK8 assays. *P<0.05.