Figure 3. Knockdown of P3H4 inhibits BC cell migration and invasion in vitro and BC growth in vivo. After siP3H4 was transfected into EJ and T24 cells, (A) the invasion ability was determined by Transwell assay, the migration ability was examined (B) and quantified (C) by wound healing assay, (D–F) the expression of EMT-related proteins was detected by western blot. (G) Images of xenograft nude mice after 4 weeks of treatment. (H) Images of subcutaneous tumor. The expression of P3H4 in subcutaneous tumors was examined by western blot (I) and IHC (J). *P < 0.05.