Figure 4. ETV4 is the transcription factor of P3H4. (A) The PROMO database predicted a variety of P3H4 transcription factors. After transfected with transcription factor overexpression plasmid respectively, P3H4 mRNA and protein expression in EJ cells were detected by RT-qPCR (B) and western blot (C). (D) After transfected with ETV4-overexpression plasmid, the expression of ETV4 and P3H4 was examined by western blot. (E) Three predicted ETV4 binding P3H4 promoter sites. (F) The site where ETV4 bound directly to the P3H4 promoter region was measured by luciferase activity assay. *P < 0.05.