Research Paper Volume 12, Issue 3 pp 2226—2245

Aucubin exerts anti-osteoporotic effects by promoting osteoblast differentiation

Figure 3. AU restored the damage of H2O2 on MG63 cells. (A) AU reduced the apoptosis rate of MG63 cells caused by H2O2. (B) AU suppressed the over-accumulation of ROS in MG63 cells caused by H2O2. (C) AU inhibited the dissipation of MMP in MG63 cells caused by H2O2. (D) AU enhanced the expression levels of Bcl-2, and reduced the expression levels of Bax and cleaved caspase-3 in MG63 cells exposed to H2O2. The quantification data of the expression levels of Bcl2, casepase3 and Bax were normalized by corresponding GAPDH. Data are expressed as mean ± S.D. (n=6) and analyzed using a one-way ANOVA. # P<0.05 and ### P<0.001 vs. control cells, *P<0.05, *P<0.01 and ***P<0.001 H2O2-exposed cells.