Research Paper Volume 12, Issue 3 pp 2246—2260

The protein tyrosine kinase inhibitor, Genistein, delays intervertebral disc degeneration in rats by inhibiting the p38 pathway-mediated inflammatory response

Figure 1. IL-1β treatment induces NPC degeneration and increases the expression of inflammatory cytokines. (A) mRNA expression of COL2A in NPCs treated with IL-1β. *p<0.05 (B) mRNA expression of aggrecan in NPCs treated with IL-1β. *p<0.05 (C) mRNA expression of collagen X in NPCs treated with IL-1β. *p<0.05 (D) Toluidine blue staining of NPCs treated with IL-1β. Black scale bar represents 100 μm. Blue scale bar represents 25 μm. (E) mRNA expression of IL-1β in NPCs treated with IL-1β. IL-1β (F) mRNA expression of TNF-α in NPCs treated with IL-1β. *p<0.05.