Research Paper Volume 12, Issue 3 pp 2246—2260

The protein tyrosine kinase inhibitor, Genistein, delays intervertebral disc degeneration in rats by inhibiting the p38 pathway-mediated inflammatory response

Figure 4. Genistein and p38 signal pathway inhibitor SB203580 attenuate the degeneration of NPCs and inhibit the phosphorylation of p38. (A) Protein expression of COL2A, aggrecan, and collagen X in NPCs treated with Genistein or SB203580. (B) Quantification of COL2A protein expression. *p<0.05 (C) Quantification of aggrecan protein expression. *p<0.05 (D) Quantification of collagen X protein expression. *p<0.05 (E) Protein expression of p38 and p-p38 in NPCs treated with Genistein or SB203580. (F) Quantification of p38 MAPK protein expression. *p<0.05 (G) Quantification of p-p38 MAPK protein expression. *p<0.05