Research Paper Volume 12, Issue 3 pp 2275—2301

PAS kinase deficiency reduces aging effects in mice

Figure 2. Effects of aging and PASK deficiency on Akt and PTEN protein levels. Immunoblot analysis of total Akt (Akt) (A), phospho-Akt (Ser473) (P-Akt) (B) and PTEN (PTEN) (C) in livers from young (3-5 months) and aged (18-20 months) wild-type (WT) and PASK-deficient (Pask-/-) mice. Liver lysates from non-fasted (NF) and 24-h fasted (F) mice were processed for western blot analysis. The value obtained for non-fasted 3-5-month-old WT mice was taken as 1. Bar graphs represent the means ± SEM of the densitometric values normalized by total protein detected by Stain-Free (TOTAL PROTEIN) (Supplementary Figure 1); n = 4-5 animals per condition. $P < 0.05, $$P < 0.01, $$$P < 0.001 3-5 months vs. 18-20 months; * P < 0.05, ** P < 0.01 WT vs. Pask-/-; #P < 0.05 non-fasted vs. fasted.