Research Paper Volume 12, Issue 3 pp 2275—2301

PAS kinase deficiency reduces aging effects in mice

Figure 3. Effects of aging and PASK deficiency on the expression of several hepatic genes involved in mitochondrial biogenesis and PGC1a and SIRT1 proteins. Real-time PCR was used to analyze the expression of Nrf2 (A), Pparg (B), Ppargc1a (C) and Sirt1 (D) genes. The mRNA levels were measured in the livers of non-fasted (NON-FASTED) and 24-h fasted (FASTED) young (3-5 months) and aged (18-20 months) wild-type (WT) and PASK-deficient (Pask-/-) mice. Immunoblot analysis of PGC1a (E) and SIRT1 (F) in livers from young and aged WT and Pask-/- mice. Liver lysates from non-fasted (NF) and 24-h fasted (F) mice were processed for western blot analysis. The value obtained in 3-5-month-old non-fasted WT mice was taken as 1. Bar graphs in (AD) represent the means ± SEM, and the levels of expression were normalized by mRNA of β-actin used as housekeeping gene;(E, F) represent the means ± SEM of the densitometric values normalized by total protein detected by Stain-Free (TOTAL PROTEIN) (Supplementary Figure 1); n = 4-5 animals per condition. $P < 0.05, $$P < 0.01, $$$P < 0.001 3-5 months vs. 18-20 months; * P < 0.05, ** P < 0.01, *** P < 0.001 WT vs. Pask-/-; #P < 0.05, ##P < 0.01, ###P < 0.001 non-fasted vs. fasted.