Figure 7. Effects of aging and PASK deficiency on the expression of several hepatic proteins involved in mitochondria remodeling and mitophagy. Real-time PCR was used to analyze the expression of fusion proteins Mfn1, Mfn2 and Opa1 (A), fission protein Fis1 (B) and mitophagy proteins Bnip3, Pink1 and Parkin (C) mRNA levels. The results were measured under non-fasted (NON-FASTED) and 24-h fasted (FASTED) conditions in livers from young (3-5 months) and aged (18-20 months) wild-type (WT) and PASK-deficient (Pask-/-) mice. The mRNA levels of different genes were normalized by the mRNA of β-actin used as housekeeping gene. The value obtained in 3-5-month-old non-fasted WT mice was taken as 1. Bar graphs represent the means ± SEM; n = 4-5 animals per condition. $ P < 0.05, $$ P < 0.01, $$$ P < 0.001 3-5 months vs. 18-20 months; * P < 0.05, ** P < 0.01 WT vs. Pask-/-; # P < 0.05, ## P < 0.01 non-fasted vs. fasted.