Figure 8. Effects of aging and PASK deficiency on MFN2, FIS1, BNIP3 and PINK1 protein expression. Immunoblot analysis of MFN2 (A), FIS1 (B), BNIP3 (C) and PINK1 (D) in livers from young (3-5 months) and aged (18-20 months) wild-type (WT) and PASK-deficient (Pask-/-) mice. Liver lysates from non-fasted (NF) mice were processed for western blot analysis. The value obtained in 3-5-month-old non-fasted WT mice was taken as 1. Bar graphs in (A–D) represent the means ± SEM of the densitometric values normalized by total protein detected by Stain-Free (TOTAL PROTEIN) (Supplementary Figure 2), n = 4-5 animals per condition. $P < 0.05 3-5 months vs. 18-20 months; * P < 0.05 WT vs. Pask-/-.