Research Paper Volume 12, Issue 3 pp 2333—2346

Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203

Figure 2. Effects of NNT-AS1 on the growth of CCLP1 and TFK1 cells, which were transfected with pcDNA-NNT-AS1 and si-NNT-AS1. (A) NNT-AS1 level was examined via qRT-PCR. (B) Proliferation was examined via BrdU. Expression of cyclinD1 was examined via western blot (C) and analyzed quantitatively (D) in both cells. (E) Apoptosis was examined via flow cytometry. Expression of cleaved-caspase-3 was examined via western blot (F) and analyzed quantitatively (G) in both cells. * P < 0.05, ** P < 0.01 and *** P < 0.001 contrasted with indicated set.