Research Paper Volume 12, Issue 3 pp 2333—2346

Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203


Figure 4. The interaction between NNT-AS1 and miR-203 or miR-203 and IGF1R/ZEB1 was examined. (A) The target matching sequence of NNT-AS1 and miR-203. (B) Luciferase activity was measured after co-transfection with NNT-AS1-wt or NNT-AS1-mut and miR-203 mimic or NC mimic. (C) Level of miR-203 was measured via qRT-PCR when cells were transfected with pcDNA-NNT-AS1 and si-NNT-AS1. (D) The target matching sequence of miR-203 and IGF1R/ZEB1. (EF) Luciferase activities were measured after co-transfection with IGF1R/ZEB1-wt or IGF1R/ZEB1-mut and miR-203 mimic or NC mimic. (GH) The mRNA and protein levels of IGF1R and ZEB1 were tested through qRT-PCR and western blot after transfection with miR-203 mimic or miR-203 inhibitor and the relative control. * P < 0.05 and ** P < 0.01 contrasted with indicated group.