Research Paper Volume 12, Issue 3 pp 2333—2346

Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203

Figure 5. Mechanism of NNT-AS1 on proliferation and EMT in CCLP1 and TFK1 cells after co-transfection with pcDNA-NNT-AS1 and miR-203 mimic. (A) Standard of miR-203 was examined via qRT-PCR by transfection with miR-203 mimic. (B) Proliferation was examined via BrdU. Expression of cyclinD1 was examined via western blot (C) and analyzed quantitatively (D) in both cells. Expression of EMT related factors was inspected via western blot (E, G) and analyzed quantitatively (F, H) in CCLP1 and TFK1 cells. * P < 0.05 and ** P < 0.01 contrasted with indicated set.