Research Paper Volume 12, Issue 3 pp 2333—2346

Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203

Figure 6. Mechanism of NNT-AS1 on PI3K/AKT and ERK1/2 passageways in CCLP1 and TFK1 cells, which were co-transfected with pcDNA-NNT-AS1 and miR-203 mimic. Expression of PI3K and AKT was measured via western blot (A, C) and analyzed quantitatively (B, D) in both cells. Expression of ERK1/2 was examined via western blot (E, G) and analyzed quantitatively (F, H) in both cells. * P < 0.05, ** P < 0.01 and *** P < 0.001 contrasted with indicated set.