Research Paper Volume 12, Issue 3 pp 2373—2392

UBE2M promotes cell proliferation via the β-catenin/cyclin D1 signaling in hepatocellular carcinoma

Figure 3. UBE2M mediates cell migration and invasion in vitro. (A) UBE2M knockdown remarkably suppressed cell migration in PLC/PRF/5 and MHCC-97L cells, while UBE2M overexpression significantly increased cell migration in SMMC-7721 and BEL-7402 cells, as demonstrated by wound-healing assay (scale bar, 50 μm). (B) Knockdown of UBE2M remarkably suppressed cell migration in PLC/PRF/5 and MHCC-97L cells, while overexpression of UBE2M significantly increased cell migration in SMMC-7721 and BEL-7402 cells, as determined by Transwell migration. Moreover, knockdown of UBE2M significantly reduced invasion capacity, while overexpression of UBE2M promoted invasion capacity, as revealed by Matrigel invasion assay (scale bar, 200 μm). Data was presented as mean ± SD (n = 3), *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test).