Research Paper Volume 12, Issue 3 pp 2393—2407

Abnormal overexpression of G9a in melanoma cells promotes cancer progression via upregulation of the Notch1 signaling pathway

Figure 1. Knockdown of G9a inhibited the proliferation and metastasis of human melanoma cells. (A) the mRNA expression of G9a was analyzed via GEPIA. The red and grey boxes represent Skin Cutaneous Melanoma (SKCM) and normal skin samples, respectively. (B) the prognostic value of G9a (EHMT2 equivalent to G9a) was analyzed via GEPIA. (C) CCK8 was performed to detect the proliferation of M14 and A375 cells transfected with two G9a specific-siRNAs (si-G9a-1 and si-G9a-2 group) or negative control siRNA (si-NC group). (D) cell proliferation was further confirmed by colony formation assay. (E) percent clone was analyzed for M14 and A375 cells. (F) transwell was performed to detect the migration and invasion of M14 cells. (G) the number of migratory and invasive cells was counted for M14 cells. (H) transwell was performed to detect the migration and invasion of A375 cells. (I) the number of migratory and invasive cells was counted for A375 cells. ** P<0.01.