Research Paper Volume 12, Issue 3 pp 2393—2407

Abnormal overexpression of G9a in melanoma cells promotes cancer progression via upregulation of the Notch1 signaling pathway

Figure 3. G9a inhibited the Notch1 pathway in melanoma and normal skin cells. (A) the correlation of G9a and Notch1 expression in SKCM samples was analyzed via GEPIA. (B) the correlation of G9a and Notch1 expression in normal skin samples was analyzed via GEPIA. (C) Western blot was performed to detect the expression of Notch1, Hes1 and H3K9me2 in G9a knockdown cells. (D) the column diagram showed the expression level of Hes1. (E) the column diagram showed the expression level of Notch1. (F) NICD was overexpressed in G9a knockdown cells (NICD group), and to detect the expression levels of p-AKT, Snail1 and NICD were detected by western blot. (G) the column diagram showed the expression level of p-AKT. (H) the column diagram showed the expression level of Snail1. (I) the column diagram showed the expression level of NICD. (J) IHC was performed to detect the expression of Notch1 and p-AKT in skin cancer tissues. (K) analysis of IHC showed the relationship between the expressions of Notch1 and p-AKT in human skin cancer. * P<0.05; ** P<0.01.