Research Paper Volume 12, Issue 3 pp 2393—2407

Abnormal overexpression of G9a in melanoma cells promotes cancer progression via upregulation of the Notch1 signaling pathway

Figure 4. UNC0642 inhibited the Notch1 signaling pathway. (A) the structural formula of UNC0642 (left panel), and the IC50 value was calculated via a dose dependent assay (right panel). (B) the expression of Notch1, Hes1 and H3K9me2 in M14 cells treated with UNC0642 or DMSO was detected with M14 cells as a blank control. (C) the expression of Notch1, Hes1 and H3K9me2 in A375 cells was detected using Western blot. (D) the column diagram showed the related expression of Notch1 in A375 and M14 cells. (E) the column diagram showed the related expression of Hes1 in A375 and M14 cells. (F) the column diagram showed the related expression of H3K9me2 in A375 and M14 cells. (G) CCK8 assay was performed to detect the proliferation of M14 cells treated with UNC0642 or DMSO. (H) CCK8 assay was performed to detect the proliferation of A375 cells treated with UNC0642 or DMSO. ** P<0.01; *** P<0.001.