Research Paper Volume 12, Issue 3 pp 2440—2452

HO-1 induced autophagy protects against IL-1 β-mediated apoptosis in human nucleus pulposus cells by inhibiting NF-κB

Figure 7. Autophagy induced by HO-1 inhibits the NF-κB pathway in NPCs. (A) Western blot analysis shows P65 and phospho-P65 protein alevels in NPCs transfected with Ad-HO-1 for 48 h and then treated with or without 10mM 3-MA for 24 h to inhibit autophagy. (B) Western blot analysis shows P65, p-P65, P62 and LC3-II/I protein levels in NPCs treated with 10mM CQ for 24 h after transfection with Ad-HO-1 for 48 h. Note: The data represent mean ± SD of three independent experiments; ****P<0.0001 and ***p<0.001.