Research Paper Volume 12, Issue 3 pp 2507—2529

PRMT5-TRIM21 interaction regulates the senescence of osteosarcoma cells by targeting the TXNIP/p21 axis

Figure 1. PRMT5 is overexpressed in OS tissues and its expression predicts poor survival probability. (A) The expression of PRMT5 was examined by IHC using commercial tissue microarrays (TMAs), which contained normal bone and different TNM stages of OS (++ indicated T1; +++ indicated T2). Representative images of PRMT5 expression in the tissues are shown. The red arrows indicate the trabecular bone. Scale bar = 50 μm. (B) The expression scores of PRMT5 in the cytoplasm and nucleus of OS cells (n = 72). (C) The expression scores of PRMT5 in normal bone (n = 27) and OS (n = 72). (D and E) PRMT5 expression along with OS survival probability was analyzed using the mixed osteosarcoma-Kuijjer dataset in the R2 Genomics Analysis and Visualization Platform (