Figure 4. Validation of altered circRNA expression by qRT-PCR. (A) The amplified circRNAs were confirmed to be head-to-tail spliced via sequencing. (B) The expression levels of differentially expressed circRNAs were determined by Real-time PCR. The Data are means ± SEM with 3 mice per group. Seq: sequence. (C) The expression of circular RNAs in human skeletal muscle cells and the liver of mice. Lane 1, 2, and 3 represented the RNA in heart, liver and human skeletal muscle cells, respectively.