Research Paper Volume 12, Issue 3 pp 2626—2646

Screening and identification of genes associated with cell proliferation in cholangiocarcinoma

Figure 6. Further expression patterns of relevant proteins via protein profiling. (A) Expression distribution of detected proteins based on screened 94 genes. (B) The 4 proteins, CENPF, COL10A1, COMP and CTHRC1, are abnormally over-expressed in tumor samples (FC > 1.3, p < 0.05). (C) For the 4 up-regulated proteins, consistent expression patterns are detected in CCA at mRNA levels. (D) Drug responses for CENPF across different cancer types.* indicates significant correlations (p < 0.05). (E) Predicted roles of the 4 genes in biological pathways (activation or inhibition). (F). RT-PCR results for screened genes in different cell lines. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 (all experiments are repeated three times). (G) The detailed expression trends for each gene based on each experimental validation. (H) The detailed expression trends for each cell line based on each experimental validation.