Uric acid induces stress resistance and extends the life span through activating the stress response factor DAF-16/FOXO and SKN-1/NRF2

Figure 2.Uric acid extends the health span of C. elegans. (A and B) Image and quantitation of the fluorescent intensity of AM140 on day 3 of adulthood. Data are the means ± SD, n ≥ 30, ** P < 0.01 (Student’s t test). (C and D) Image and quantitation of the polyQ puncta aggregates of AM140 on day 3 of adulthood. Data are the means ± SD, n ≥ 30, * P < 0.05 (Student’s t test). (E) Western blot analysis of polyQ35::YFP in the presence and absence of 2 mM uric acid at day 3 of adulthood. (F) mRNA level of Q35::YFP when animals treated with or without 2 mM uric acid on day 3 of adulthood (means ± SD, n = 3, n.s.: no significant difference, Student’s t test). (G) Effects of 2 mM uric acid on pharyngeal pumping. * P < 0.05 for uric acid versus control at day 1 and day 5 adulthood. Data are the means ± SD, n = 20-30 animals (there were fewer animals on later days), Student’s t test. (H) Effects of 2 mM uric acid on period of fast movement (means ± SD, n ≥ 60, * P < 0.05, Student’s t test).