Research Paper Volume 12, Issue 3 pp 2880—2896

In vivo protective effect of adipsin-deficiency on spontaneous knee osteoarthritis in aging mice

Figure 1. Micro-CT (μCT) and histology of joint tissues of 20-month-old mice. (A) Knee joint μCT of adipsin-deficient (Df-/-), and the wild-type Df+/+(L) and (H) mice. Representative 3-dimensional reconstructions of the joint and axial and coronal images of the subchondral bone compartment. Black arrows indicate osteophytes, white arrows sclerosis and the white arrowheads joint space in the medial compartment. (B) Photomicrographs of representative histological sections of joint tissues: cartilage, meniscus and subchondral bone. The dotted lines delineate subchondral plate thickness and the black arrowheads cartilage alterations. Bar = 100 μm. Original magnification X63. (CJ) Representative box plots of the Osteoarthritis Research Society International (OARSI) score of the (C) medial tibial plateau, and (D) medial femoral condyle; the (E) anterior and (F) posterior menisci; the subchondral bone assessment of the (G) percentage of bone (% bone volume [BV]/total volume [TV]), (H) trabecular (tb) thickness, (I) plate thickness, and (J) tartrate resistant acid phosphatase (TRAP) assay. (K) Photomicrographs of representative histological sections of the synovial membrane and (L) box plot of the OARSI score of the synovial membrane. In (K) the dotted lines delineate synovial membrane thickness. Bar = 100 μm. Original magnification X100. For each box plot, values are the median and interquartile range of Df-/- (n=13), Df+/+ (n=13), Df+/+(L) (n=7) and Df+/+(H) (n=6). p values were determined by the Mann-Whitney test and only significant values are shown.