Research Paper Volume 12, Issue 3 pp 2921—2938

The nuclear localization signal-mediated nuclear targeting of herpes simplex virus 1 early protein UL2 is important for efficient viral production

Figure 3. Characterization of the functional NLS and its key aa of UL2. (A) Schematic representation of WT UL2 and its deletion mutants UL2(1-68), UL2(1-31), UL2(18-31), UL2(1-17), UL2(1-8), UL2(9-17) and UL2(12-14)mut fused with EYFP. (BD) Subcellular localization of these UL2 deletion mutants (B and D, shown in A), EYFP-ECRF4, or UL2(NLS)-EYFP-ECRF4 (C) in plasmid-transfected live COS-7 cells. All scale bars indicate 10 μm.