Figure 3. Characterization of the functional NLS and its key aa of UL2. (A) Schematic representation of WT UL2 and its deletion mutants UL2(1-68), UL2(1-31), UL2(18-31), UL2(1-17), UL2(1-8), UL2(9-17) and UL2(12-14)mut fused with EYFP. (B–D) Subcellular localization of these UL2 deletion mutants (B and D, shown in A), EYFP-ECRF4, or UL2(NLS)-EYFP-ECRF4 (C) in plasmid-transfected live COS-7 cells. All scale bars indicate 10 μm.