Research Paper Volume 12, Issue 3 pp 2921—2938

The nuclear localization signal-mediated nuclear targeting of herpes simplex virus 1 early protein UL2 is important for efficient viral production

Figure 6. Protein expression and subcellular localization of UL2 in different recombinant viruses-infected cells. (A) Monolayer HEK293T cells were infected with different reconstitute virus vUL2, vUL2Del, vUL2Mu or vUL2Rev at an MOI of 1 for 24 h, and cells were harvested when CPE reached 90-95%. Cell lysates were then subjected to WB analysis using the prepared anti-UL2 pAb and AP-conjugated goat anti-rat IgG. (B) Vero cells infected with different reconstitute virus vUL2, vUL2Del, vUL2Mu or vUL2Rev at an MOI of 1 for 24 h, then cells were subjected to IFA using anti-UL2 pAb and Dylight 649 conjugated goat anti-rat IgG, to show the subcellular localization of UL2. Cells were finally counterstained with Hoechst to visualize the nuclei. GFP was also captured to show the cells were successfully infected by HSV-1. All scale bars indicate 10 μm.