Research Paper Volume 12, Issue 4 pp 3156—3174

LncRNA MEG3 targeting miR-424-5p via MAPK signaling pathway mediates neuronal apoptosis in ischemic stroke

Figure 1. MRNA Sema3A was highly expressed in IS and correlated with cell viability and apoptosis. (A) The mRNA level of Sema3A in N2a cells climbed up with time after 6h, 12h, 24h of OGD/R treatment. *P<0.05 vs control. (B) Successful downregulation of Sema3A by si-Sema3A. *P<0.05 vs control. (C) Sema3A downregulation led to higher viability of OGD/R cells. *P<0.05 vs control, #P<0.05 vs siRNA-NC. (D, E) OGD/R cells have higher apoptosis rate compared with control groups while Sema3A downregulation is correlated with lower apoptosis level among OGD/R cells. *P<0.05 vs control, #P<0.05 vs siRNA-NC. (F) OGD/R cells tend to have higher level of c-caspase-3 as well as p-JNK and p-p38 in comparison with control group. Downregulation of Sema3A could counteract with this trend in OGD/R cells partly. *P<0.05 vs control, #P<0.05 vs siRNA-NC.