Research Paper Volume 12, Issue 3 pp 2974—2991

Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis

Figure 6. MYLIP is a direct target of miR-190b-5p in breast cancer cells. (A) Complementary sequence between miR-190b-5p and wild type (wt) 3′UTR of MYLIP. The putative binding sites of miR-190b-5p was mutated in 3′UTR of MYLIP (mut MYLIP-3′UTR). (B) SK-BR-3 cells that were co-transfected with miR-190b-5p mimics and wt or mut 3′UTR of MYLIP were subjected to luciferase activity measurement. (C) SK-BR-3 cells that were transfected with miR-190b-5p mimics or miR-190b-5p inhibitors were subjected to qRT-PCR for MYLIP mRNA expression. (D) MDA-MB-435 cells that were co-transfected with miR-190b-5p mimics and wt or mut 3′UTR of MYLIP were subjected to luciferase activity measurement. (E) MDA-MB-435 cells that were transfected with miR-190b-5p mimics or miR-190b-5p inhibitors were subjected to qRT-PCR for MYLIP mRNA expression. (F) miR-190b-5p overexpression reduced the protein expression level of MYLIP in MDA-MB-435 cells. (G) miR-190b-5p inhibition increased the protein expression level of MYLIP in SK-BR-3 cells. The asterisk (*) indicates a significant difference (p < 0.05).