Research Paper Volume 12, Issue 3 pp 2992—3009

EGF released from human placental mesenchymal stem cells improves premature ovarian insufficiency via NRF2/HO-1 activation

Figure 4. EGF derived from hPMSCs improved the proliferation rate and inhibited the apoptosis rate in POI hGCs. (A) FACS results indicated that hPMSCs, hPMSC-CM or EGF treatment improved the proliferation rate (Ki67) in POI hGCs. (B) FACS results indicated that hPMSCs, hPMSC-CM or EGF treatment inhibited the rate of apoptosis (Annexin V) in POI hGCs. (C) Western blot results demonstrated that hPMSCs, hPMSC-CM or EGF treatment increased the expression of apoptosis resistance genes (Bcl2 and Survivin) and reduced the expression levels of apoptosis genes (Caspase 3 and Caspase 9). All of the experiments were carried out three times; the error bars indicate the SD; *** p < 0.001 (compared with the POI group). POI = premature ovarian insufficiency.