Research Paper Volume 12, Issue 3 pp 2992—3009

EGF released from human placental mesenchymal stem cells improves premature ovarian insufficiency via NRF2/HO-1 activation

Figure 8. EGF affected ROS levels by regulating the NRF2/HO-1 signaling pathway but not the PI3K/AKT signaling pathway. (A) qPCR analysis of the expression levels of PTEN, NRF2, HO-1, PI3K and AKT in hGCs-NRF2KD after treatment with EGF. (B) Western blot analysis of the expression levels of PTEN, NRF2, HO-1, PI3K and AKT in hGCs-NRF2KD after treatment with EGF. (C) ROS levels were measured in hGCs-NRF2KD after treatment with hPMSCs and EGF. (D) qPCR analysis of the expression levels of PTEN, NRF2, HO-1, PI3K and AKT in hGCs-PTENKD after treatment with EGF. (E) Western blot analysis of the expression levels of PTEN, NRF2, HO-1, PI3K and AKT in hGCs-PTENKD after treatment with EGF. (F) ROS levels were measured in hGCs-PTENKD after treatment with hPMSCs and EGF. All experiments were carried out three times. The error bars indicate the SD; **, p < 0.01; *** p < 0.001 (compared with the NRF2 or PTEN knockdown group, respectively). The qPCR primer sequences used are listed in Supplementary Table 1.