Research Paper Volume 12, Issue 3 pp 3010—3024

microRNA-1203 targets and silences cyclophilin D to protect human endometrial cells from oxygen and glucose deprivation-re-oxygenation

Figure 2. miR-1203 inhibition can elevate CypD expression in human endometrial cells. T-HESC endometrial cells were infected with pre-miR-1203 anti-sense lentivirus (“lv-antagomiR-1203”), following puromycin selection two stable cell lines were established: “L1/L2”. Control T-HESC cells were infected with microRNA anti-sense control lentivirus (“antaC”); Expression of mature miR-1203 and CypDmRNA was tested by qPCR assays (A and C); The relative CypD 3’-UTR luciferase reporter activity was examined (B), with CypD protein expression tested by Western blotting (D). The primary human endometrial cells were infected with lv-antagomiR-1203 or antaC for 48h, expression of mature miR-1203 (E), CypDmRNA (F) and protein (G) was shown. CypD protein expression was quantified and normalized to the loading control (D and G). Data were presented as mean ± SD (n=5), and results were normalized. * P <0.05 vs. “Vec”/“antaC” cells. Experiments in this figure were repeated five times with similar results obtained.