Research Paper Volume 12, Issue 3 pp 3025—3041

Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma

Figure 1. Upregulation of OLA1 in hepatocellular carcinoma (HCC). (A) The Cancer Genome Atlas (TCGA) liver cancer dataset (liver hepatocellular carcinoma) included the messenger RNA (mRNA) expression of OLA1 in HCC tissues (n = 371) and normal tissues (n = 50). (B) OLA1 was highly expressed in HCC tissues compared with normal liver tissues according to the analysis of GEO datasets. GSE6764, tumor, n=35; normal, n=23. GSE29721, tumor, n=10; normal, n=10. GSE45436, tumor, n=95; normal, n=39. GSE62232, tumor, n=81; normal, n=10. (C) Real-time PCR analysis of OLA1 expression in 20 pairs of HCC specimens and corresponding normal liver tissues (P<0.01). (D) OLA1 is upregulated in HCC tissues, as shown by western blot results. (E) OLA1 is upregulated in 70 out of 105 HCC tissues, as shown by IHC. (F) Representative images of OLA1 staining in HCC specimens and normal liver tissues. P, patient; T, tumor; N, normal.