Research Paper Volume 12, Issue 3 pp 3025—3041

Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma

Figure 3. Inhibition of OLA1 represses the proliferation, migration, invasion and tumorigenicity of HCC cells. (A and B) OLA1 was highly expressed in LM3 and MHCC-97H cells and inhibited by transfection with OLA1-siRNA. (C) OLA1 silencing repressed the proliferation of HCC cells. (D) OLA1 downregulation inhibited cell migration after transfection. (E) The transwell Matrigel penetration assay showed that OLA1 promoted the invasion of HCC cells. (FH) OLA1 silencing significantly reduced tumor growth and tumor weight in xenografts carrying HCC cells. (I) Ki-67 was significantly downregulated in OLA1-knockout tumors, as shown by IHC. All *p<0.05, **p<0.01, ***p<0.001.