Research Paper Volume 12, Issue 4 pp 3218—3237

LINC00623/miR-101/HRAS axis modulates IL-1β-mediated ECM degradation, apoptosis and senescence of osteoarthritis chondrocytes

Figure 6. LINC00623/miR-101/HRAS axis modulates the apoptosis, senescence and ECM degradation in OA chondrocytes through MAPK signaling pathway. Chondrocytes were co-transfected with miR-101 inhibitor and sh-LINC00623 and expose to IL-1β stimulation; the protein levels of HRAS, ERK, p-ERK, MMP13, Collagen II, Caspase 3, cleaved-Caspase 3, Caspase 7 and cleaved-Caspase 7 were examined using immunoblotting (A); the localization of Collagen II was examined using IF (B); the expression levels of LINC00623 was determined by real-time PCR (C); the activity of MMP-13 was determined by a SensoLytes Plus 520 MMP13 assay kit (D); the SA-β-Gal positive cells were determined by the SA-β-Gal staining (E); the cell apoptosis was examined using flow cytometry (F) and TUNEL (G). The data are presented as mean ± SD of three independent experiments. *P<0.05, **P<0.01, compared to control group; #P<0.05, ##P<0.01, compared to sh-LINC00623 group.