Research Paper Volume 12, Issue 5 pp 4093—4110

Exosomal miR-19a from adipose-derived stem cells suppresses differentiation of corneal keratocytes into myofibroblasts

Figure 1. FBS induces differentiation of rabbit corneal keratocytes into myofibroblasts. (A) Representative immunofluorescence images show staining of rabbit corneal stromal cells with fluorescent-tagged antibodies against Vimentin (red) and CK12 (green). Vimentin-positive cells are the keratocytes; CK12-positive cells are the corneal epithelial cells; Diamidinophenylindole (DAPI; blue) stains the nucleus; magnification: 200x. (B) Representative phase-contrast images show phenotypic features of rabbit corneal keratocytes cultured in 10% FBS or serum-free DMEM/F12 medium for 1, 3, 5 and 7 days. The cell cultures were observed under a light microscope and images were captured at 10X magnification. (C) Representative western blot images show levels of keratocan, collagen I, collagen III, MMP9, fibronectin and α-SMA proteins in rabbit keratocyte cells grown in DMEM/F12 medium containing 10% FBS or serum-free medium for 7 days. GAPDH was used as an internal control. (D) Histogram plot shows keratocan, collagen I, collagen III, MMP9, fibronectin and α-SMA protein levels relative to GAPDH. ** denotes P < 0.01 compared with the control (1d) group; ## denotes P < 0.01 compared with the 10% FBS (1d) group.