Research Paper Volume 12, Issue 5 pp 4093—4110

Exosomal miR-19a from adipose-derived stem cells suppresses differentiation of corneal keratocytes into myofibroblasts

Figure 3. ADSCs-Exo inhibits FBS-induced differentiation of keratocytes into myofibroblasts. (A) CCK-8 assay results show the viability of rabbit corneal keratocytes incubated with ADSCs or ADSCs-Exo in the presence of 10% FBS for 0, 24, 48, 72 and 96 h. (B) Representative western blotting images show levels of keratocan, α-SMA, MMP9, collagen I, collagen III, and fibronectin proteins in rabbit corneal keratocytes incubated with ADSCs or ADSCs-Exo in the presence of 10% FBS for 96 h. GAPDH was used as an internal control. (C, D) Histogram plots show the levels of keratocan, α-SMA, MMP9, collagen I, collagen III, and fibronectin proteins relative to GAPDH in rabbit corneal keratocytes incubated with ADSCs or ADSCs-Exo in the presence of 10% FBS for 96 h, as determined by western blotting. ** denotes P < 0.01 as compared with the control group; ## denotes P < 0.01as compared with the 10% FBS group.