Research Paper Volume 12, Issue 4 pp 3238—3248

Peritoneal metastatic gastric carcinoma cells exhibit more malignant behavior when co-cultured with HMrSV5 cells

Figure 5. Metastatic gastric cancer cell supernatants significantly induced the EMT in peritoneal mesothelial cells. (A, B) HMrSV5 cells were co-cultured with NUGC-4, NUGC-4per, NUGC-4liver and NUGC-4lym cell supernatants for 72 h. Then, the relative levels of E-cadherin, N-cadherin, α-SMA, Snail, Twist, Slug, MMP3, MMP9 and Cyclin D1 in HMrSV5 cells were detected by Western blotting. **P<0.01 compared to the control group.