Figure 4. Analysis of claudin4/18 expression and localization. (A, B) The expression of claudin4 expression was significantly up-regulated while the expression of claudin18 was significantly down-regulated in the IL-1β group. &, P < 0.01. (C) Claudin4 immunofluorescence was increased significantly in the cell membrane localization. (D) Claudin18 immunofluorescence results was reduced significantly in cell membrane localization.